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Clement Gautier, Ph.D.
cgautier@rics.bwh.harvard.edu
(617)525-5261

BIOGRAPHICAL SKETCH

POSITION TITLE: Research Fellow

EDUCATION:

2005 Ph.D. in Biochemistry/Biophysics, Ecole Polytechnique (France).
Project: Development of a brand new family of photoactivable chromophores to replace the NADPH as the first electron donor in NO-Synthases. These chromophores allowed us to trigger the electron transfer with a pump laser and follow the first steps of electron transfer through the protein via the absorption of a second laser beam.
   
2002 M.Sc. in Biophysics, Ecole Polytechnique (France). Project : Study of dynamic of geminate recombination of NO to NO-synthase on picosecond time scales.
   
2002 Master of Engineering: ENSA Montpellier (France)
   

PROFESSIONAL EXPERIENCE:

2001 Research Trainee at the Department of Biology-
Massachusetts Institute of Technology, Cambridge, MA.
   
2006-present Research Fellow-
Center for Neurologic Diseases, Brigham and Women’s Hospital


PUBLICATIONS:

1.) Gautier C, Negrerie M, Wang Z. Q., Lambry JC, Stuehr D. J., Collin F, Martin JL, Slama-Schwok A. “Dynamic regulation of the inducible nitric-oxide synthase by NO: comparison with the endothelial isoform”. J. Biol. Chem. (2004) ; 279 (6), 4358-4365

2.) Gautier C, van Faassen E, Mikula I, Martasek P, Slama-Schwok A. Endothelial nitric oxide synthase reduces nitrite anions to NO under anoxia. Biochem. Biophys. Res. Commun. (2006) 341 (3) 816-821.

3.) Gautier C, Mikula I, Nioche P, Martasek P, Raman CS, Slama-Schwok A. “Dynamics of NO rebinding to the heme domain of NO synthase-like proteins from bacterial pathogens.” Nitric Oxide. (2006) 15 (4) 312-327.

4.) Hunt D.E , Klepac-Ceraj V, Acinas- Gonzales S, Gautier C, S. Bertilson S. , Polz M.F. “Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity”. Appl Environ Microbiol. (2006) 72 (3) 2221-2225

5.) Robin A-C, Gmouh S, Mongin O, Jouikov V, Werts M.H.V,Gautier C, Slama-Schwok A and Blanchard-Desce M “A NADPH substitute for selective photo-initiation of reductive bioprocesses via two-photon induced electron transfer” Chem. Commun., (2007)

6.) Beaumont E, Lambry JC, Gautier C, Robin AC, Gmouh S, Berka V, Tsai AL, Blanchard-Desce M, Slama-Schwok A. Synchronous Photoinitiation of Endothelial NO Synthase Activity by a Nanotrigger Targeted at Its NADPH Site. J Am Chem Soc. (2007)

PATENT:

Slama-Schwok A, Gautier C, Martin JL, Gmouh S, Blanchard-Desce M“Photoactivable NADPH analog for synchronous trigger of enzymatic catalysis” Fr. appl n° 05/11914, 11/23/05.

 

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